How a Blood Typing Test is Done
1. In a blood typing tray, place a few drops of someone's blood into each well. This is where we will test for A antigens, B antigens, and the Rh factor. (We will not test for Rh factor in today's lab, but that is where the + or - label comes from in blood types). 2. Place a drop of "anti-A" serum in well A. 3. Place a drop of "anti-B" serum in well B. 4. Stir each with a clean toothpick, do not cross-contaminate! 5. Observe. If the antigens are present, the blood will react with the anti-A or anti-B solution. You will see some separation and clumping showing the proteins are present. |
How to Read a Blood Typing Tray
The sample above shows there are antigens in well B. This sample is positive for B antigens, but does not have A or Rh factor. This person would have the blood type B-.
The sample above shows there are antigens in well B. This sample is positive for B antigens, but does not have A or Rh factor. This person would have the blood type B-.